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Vývoj protilátkové imunitní odpovědi proti kryptosporidiovým infekcím
FENCLOVÁ, Jana
This bachelor thesis is focused on the development of humoral immune response against cryptosporidial infections in BALB/c mice. Mice were infected with various Cryptosporidium spp., namely C. parvum, C. proliferans, C. baileyi and C. andersoni. Cryptosporidium-specific IgG antibodies were detected by ELISA. In this work, cross-reactivity of antibodies with different Cryptosporidium species was also monitored.
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Postnatální změny vybraných krevních parametrů
TRUHLÁŘOVÁ, Nela
The aim of the bachelor thesis was to evaluate changes in hematological parameters in the postnatal period of calves. The literature review describes blood composition, physical properties of blood, hematological parameters and cryptosporidia, which are occurs commonly found in calves. We included 18 calves of holstein cattle and czech calves in the experiments red-spotted cattle, which we divided into two groups according to age. Observation started at 20 days of calf age and ended at 300 days of calf age. The results show that that hematological parameters change in postnatal calves. Concentration erythrocytes increased from birth to 140 days of age, but then with increasing age of calves there was a gradual decrease in the concentration of erythrocytes (from 10,35 × 1012/L at 7,45 × 1012/L).The hematocrit value (from 0,34 l/l to 0,27 l/l), hemoglobin concentration (from 133 ? g/l to 106 ? g/l) and mean erythrocyte volume had similar dynamics, (from 35,57 ? 1,44 fl to 33,12 fl). The leukocyte count in group 2 was in the refer-ence range for adult cattle (8,68 ? to 12,96 ? G/l), but in group 1 (c. parvum, c. bo-vis) they exceeded the reference range, especially in the period between 160.-180. bot-tom (12,96 G/l). The most stable percentage was lymphocytes, which accounted for 59,55 to 78,26% of the total number. In contrast, neutrophilic granulocytes had a signifi-cant tendency to decrease (from 31,70% to 14,53%). Anyway thus, there was a gradual percentage decrease in monocytes (from 9,17% to 7,51%). Platelet counts fluctuated slightly, but the average proportion ranged between 631,83-652,21 G/l.
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Kryptosporidie a kryptosporidióza chovaných a zdivočelých nutrií
LIMPOUCHOVÁ, Zlata
Cryptosporidium spp., common parasite of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutria (Myocastor coypus). A total of 247 faecal samples of feral and farmed nutrias were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci.
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Seroprevalence of Cryptosporidium spp. in the human population
KUTSYNA, Valeriy
In 2018 - 2020, 1366 samples of serum from immunocompetent patients, including 860 sera from the Czech Republic and 506 sera from Ukraine, were collected and examined by an indirect ELISA test to detect specific IgG antibodies against Cryptosporidium in human population. Age, sex, and relevant data on previous cryptosporidial infections were obtained from each patient. Oocysts of C. parvum, 105 oocysts per well, were used as a corpuscular antigen. Blood serum samples and secondary antibodies were diluted 1:200 and 1: 20,000, respectively. The age range of patients varied from newborn infant to 89 years old. A total of 639 male and 709 female patients were examined. The resutts showed number of serologically positive patients increased with age. Moreover, females had higher seropositivity than males, regardless of nationality. Additionally, Czechs were more often positive than Ukrainians. The results showed less than 20 % of patients under 15 years of age were seropositive. In patients older than 15 years the seroprevalence ranged from 20 to 42 %, except the age group 41 - 50 years old, where seroprevalence was 50 %.
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Střevní paraziti ptáků na Svalbardu
ŽÁNOVÁ, Veronika
The study aimed to obtain essential knowledge about the distribution of intestinal parasites of birds occurring in Svalbard. Noninvasive coprological techniques were used to detect coccidian, cryptosporidian and microspordian parasites. The research included field collection of faecal samples and laboratory examination by microscopic and molecular methods.
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Diverzita kryptosporidií u pěvců
LIMPOUCHOVÁ, Zlata
This study was aimed on research of biology and diversity of Cryptosporidium in perching birds (Passeriformes). Presence of Cryptosporidium oocyst and specific DNA in faecal samples was detected by microscopy and molecular methods using nested PCR. Biological properties and intensity of infection of Cryptosporidium were examined and described in both experimentally and naturally infected animals.
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Střevní paraziti savců introdukovaných na Svalbard
BROŽ, Marek
The aim of this thesis was the research of parasites of mammals introduced to Svalbard: sibling voles, horses and dogs. Faeces of this three species were used for examination of intestinal parasites. Samples were collected in central part of Svalbard and examined in Czech Republic by microscopic and molecular methods for detection of protists and helminths.
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Fox cryptosporidiosis
POKORNÝ, Radek
Foxes represent a reservoir of zoonotic diseases and it is necessary perform a control of disease situation. Total 111 fecal samples originated form wild foxes were collected in Czech Republic and Poland in two consequence years 2013 - 2014. All fecal samples were screened for presence of Cryptosporidium spp. using both microscopy and molecular methods. Altogether two samples were molecularly positive and phylogenetic analyses reveal presence C. andersoni in both of them. Low prevalence (1.8%) and low infection intensity was observed in this study. Eighteen cases of diarrhea were detected, but no animal was positive for cryptosporidium infection.
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The role of Felidae in the environmental dissemination of human pathogenic unicelular parasites
GRYM, Ondřej
One hundred cats at different levels of habituation, house kept and free living, from Czech republic were screened for the presence of Cryptosporidium, Encephalitozoon spp. and Enterocytozoon bieneusi to consider the risk of close human - cat contact on cryptosporidial and microsporidial infections. The prevalence of Cryptosporidium, Encephalitozoon, and Enterocytozoon bieneusi in cats was determined from fecal specimens by genus-specific PCR. All PCR-positive specimens were sequenced to determine the genotype present. Out of 100 cats, three (3 %) were positive for Cryptosporidium, namely C. felis. Nine cats (9 %) were infected with E. bieneusi genotype D. None out of 35 house kept cats was infected with any tested parasites. No effect of age of animals on occurrence both Cryptosporidium and microsporidia was proved. To our knowledge, this is the first report on the presence of zoonotic species/genotypes of Cryptosporidium, and E. bieneusi in cats in the Czech republic.
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